Description
Aviva Systems Biology Gnb3 ELISA Kit (Mouse) (OKEH06587) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Gnb3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gnb3 and incubated. The Gnb3 found in the sample or standards competes with the biotinylated Gnb3 for limited binding sites on the immobilized anti-Gnb3 antibody. Excess unbound biotinylated Gnb3 and sample or standard Gnb3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gnb3 captured in the well and inversely proportional to the amount of Gnb3 which was contained in the sample or standard